PCM1 Knockout cell line (HeLa)

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location： Home > Products > KO Cell Lines > PCM1 Knockout cell line (HeLa)

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PCM1 Knockout cell line (HeLa) Order

PCM1 Knockout cell line (HeLa)
Catalog#
YKO-HS11544
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PCM1 Knockout cell line (HeLa)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
PCM1 Knockout cell line (HeLa)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:6
Species
Human
Gene Name
PCM1
Gene ID
5108
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS
Target region
Knockout region: Exon 4 (based on transcript PCM1-201)
Validation: PCR+Sanger Sequencing
PCM1 Knockout cell line (HeLa) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:HeLa		 STR Info (Cell bank)
Cell Line:HELA
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 1213.31213.3
D7S820 812812
D16S539 910910
VWA 16181618
TH01 7777
AMEL XXXX
TPOX 812812
CSF1PO 910910
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PCM1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HeLa cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PCM1 gene knockout PCM1 Knockout cell line (HeLa) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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